Enhanced Green Fluorescent Protein (EGFP) | 4999

The recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The recombinant EGFP (UniProt Accession No. P42212) is a 32.7 kDa monomer with 293 amino acids tagged with 6XHis on both N- and C-terminus. Therefore, the EGFP can be detected by both His-tag antibody (Cat. No. 3998-100) and GFP antibody (Cat. No. 3999-100) on Western Blot analysis. The excitation and emmission spectra for the recombinant EGFP is identical to GFP purified from Aequorea victoria (Chalfie et al, 1994), with Ex/Em = 488/507 nm (e489 nm = 55,000 M-1 Cm-1; e280 nm = 21980 M-1 Cm-1). 

4999 | Enhanced Green Fluorescent Protein EGFP DataSheet

Biomolecule/Target: N/A

Synonyms: Recombinant EGFP Protein, Enhanced Green Fluorescent Protein

Alternates names: IL-TIF, TIFa, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23

Taglines: Fluorescent protein ideal for subcellular labeling/visualization

NCBI Gene ID #: 50616

NCBI Gene Symbol: IL22

Gene Source: N/A

Accession #: Q9GZX6

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: 97%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Recombinant IL-22 activates STAT following receptor-ligand interaction.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 16.8 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Freeze Dried

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum. This solution can then be stored at 4°C for 1 week or -20° for future use.

Amino acid sequence: N/A