ENPP2 Antibody (Center) [APR07713G]

ENPP2 Antibody (Center) [APR07713G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This ENPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the Central region of human ENPP2.

Cellular Localisation: Secreted

Molecular Weight: 98994

Clone: Polyclonal

Gene Name: ENPP2

Gene ID: 5168

Function: Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931, PubMed:14500380, PubMed:12354767, ) . Major substrate is lysophosphatidylcholine (PubMed:12176993, PubMed:27754931, PubMed:14500380) . Also can act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility (PubMed:14500380) . Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:15769751, PubMed:12176993) . Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed:11559573) . Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed:1733949) . May have a role in induction of parturition (PubMed:12176993) . Possible involvement in cell proliferation and adipose tissue development (Probable) . Tumor cell motility-stimulating factor (PubMed:1733949, PubMed:11559573) . Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids (PubMed:21393252) . Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3- phosphocholine (lyso-PAF) (PubMed:21393252) .

Summary: Tissue Location: Detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182) . Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS) Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: FC, WB

Dilution: WB--1:2000

Synonyms: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, E-NPP 2, Autotaxin, Extracellular lysophospholipase D, LysoPLD, ENPP2, ATX, PDNP2