Enterokinase, human recombinant | 7136

Proteases (also called Proteolytic Enzymes, Peptidases, or Proteinases) are enzymes that hydrolyze the amide bonds within proteins or peptides. Most proteases act in a specific manner, hydrolyzing bonds at or adjacent to specific residues or a specific sequence of residues contained within the substrate protein or peptide. Proteases play an important role in most diseases and biological processes including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins. Enterokinase sequentially cleaves carboxyl side of D-D-D-D-K. Human Enterokinase is expressed as a linear 1019 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of Enterokinase, which consists of two polypeptide chains (heavy chain and light chain) held together by a single disulfide bond, resulting in formation of a biologically active heterodimer. The heavy chain consists of 784 amino acid residues, and the light consists of 235 amino acid residues.

7136 | Enterokinase human recombinant DataSheet

Biomolecule/Target: Enterokinase

Synonyms: Serine protease 7, transmembrane protease serine 15, Enteropeptidase

Alternates names: IL-TIF, TIFa, IL-10-related T-cell-derived-inducible factor, IL-22, ILTIF, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23

Taglines: Hydrolyzes the amide bonds within proteins or peptides

NCBI Gene ID #: 50616

NCBI Gene Symbol: IL22

Gene Source: Human

Accession #: Q9GZX6

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.2 ng/g of protein (<2EU/g).

Activity (Specifications/test method): Sequentially cleaves carboxyl side of D-D-D-D-K.

Biological activity: Recombinant IL-22 activates STAT following receptor-ligand interaction.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 16.8 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.5 and 1 mM Calcium Chloride.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum. This solution can then be stored at 4°C for 1 week or -20° for future use.

Amino acid sequence: N/A