65

EnzyChrom™ Aspartate Transaminase Assay Kit

(No reviews yet) Write a Review
SKU:
65-EASTR-100-GEN
Availability:
In Stock
£1,741.60

Description

EnzyChrom™ Aspartate Transaminase Assay Kit

 

Application: For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity.

Key Features:

  • Sensitive. Linear detection range: 2-100 U/L.
  • Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min.

Method: OD340nm

Samples: Serum, plasma etc

Species: All

Size: 100 tests

Detection Limit: 2 U/L

Shelf Life: 6 months

More Details:

Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and a-ketoglutarate to oxaloacetate and glutamate. There are two isoenzymes in humans: GOT1 is a cytosolic isoenzyme derived from red blood cells and heart; GOT2 is the mitochondrial isoenzyme found mainly in the liver.

AST is elevated in liver and muscle diseases. It is part of diagnostic tests for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anemia, severe burns, acute renal disease and trauma. Simple, direct and automation-ready procedures for measuring AST activity find wide applications in research and drug discovery. Gentaur Genprice AST activity assay is based on the quantification of oxaloacetate produced by AST. In this assay, oxaloacetate and NADH are converted to malate and NAD by the enzyme malate dehydrogenase. The decrease in NADH absorbance at 340 nm is proportionate to AST activity.

View AllClose

Additional Information

Usage:
Quantitative determination of aspartate transaminase activity at 340nm.
Procedure:
10 min
Size:
100 Tests
Detection limit:
2 U/L.
Shelf life:
6 months.
Shipping:
on ice
Storage:
-20°C.
View AllClose