65

EnzyChrom™ D-Lactate Assay Kit

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SKU:
65-EDLC-100-GEN
Availability:
In Stock
zł5,527.20

Description

EnzyChrom™ D-Lactate Assay Kit

 

Application: For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

Key Features:

  • Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method: OD565 nm

Samples: Serum, plasma, cell culture media etc

Species: All

Size: 100 tests

Detection Limit: 0.05 mM

Shelf Life: 6 months

More Details:

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.

D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

Gentaur Genprice EnzyChrom™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

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Additional Information

Usage:
Quantitative determination of D-lactic acid by colorimetric (565nm) method.
Procedure:
20 min
Size:
100 Tests
Detection limit:
0.05 mM.
Shelf life:
6 months.
Shipping:
on ice; Storage= -20°C."
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