EnzyChrom™ Free Fatty Acid Assay Kit

Application: For quantitative determination of fatty acid and evaluation of drug effects on its metabolism.

Key Features:

• Sensitive. Use 10 μL samples. Linear detection range: colorimetric assay 7 - 1000 μM, fluorimetric assay 7 - 100 μM fatty acid.
• Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day.

Method: OD570nm, or FL530/585nm

Samples: Serum, plasma, urine, saliva, milk, cell cultures, food, agriculture etc

Species: All

Size: 100 tests

Detection Limit: 7 μM

Shelf Life: 6 months

More Details:

Fatty acids are aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil and wax. Fatty Acids play important roles in cellular synthesis, energy metabolism and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome and Reye Syndrome. Gentaur Genprice method provides a simple, one-step and high-throughput assay for measuring free fatty acids.

In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to H₂O₂. The resulting H₂O₂ reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the free fatty acid concentration in the sample.