EnzyChrom™ NADP/NADPH Assay Kit

Application: Direct Assays: NADP+/NADPH concentrations and ratios in cell or tissue extracts. (This kit replaces ECNP-100, which has been discontinued.)

Key Features:

• Sensitive and accurate. Detection limit 0.1 µM, linearity up to 10 µM NADP+/NADPH in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method: Colorimetric Determination at 565 nm

Samples: Cells, Tissue

Species: human, mouse, rat and more

Size: 100 tests

Detection Limit: 0.1 μM

Shelf Life: 6 months

More Details:

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.

Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. Gentaur Genprice' EnzyChrom™ NADP+ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and is not interfered by NAD+/NADH. Our assay is a convenient method to measure NADP, NADPH and their ratio.