EnzyFluo™ NAD/NADH Assay Kit

Application: For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. Direct NAD/NADH Assays in 96-Well Plate^NEW!!!

Key Features:

• Sensitive and accurate. Detection limit of 0.02 μM and linearity up to 1 μM NAD+/NADH in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method: F530/585nm

Samples: Cell, tissue extracts etc Direct NAD/NADH Assays in 96-Well Plate^NEW!!!

Species: All

Size: 100 tests

Detection Limit: 0.02 μM

Shelf Life: 6 months

More Details:

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD⁺/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue.

Gentaur Genprice EnzyFluo™ NAD⁺/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NAD⁺/NADH concentration in the sample.

This assay is highly specific for NAD⁺/NADH with minimal interference (<1%) by NADP⁺/NADPH and is a convenient method to measure NAD, NADH and their ratio.