Eotaxin-3/CCL26, human recombinant | 7138

Eotaxin-3 is a CC chemokine that signals through the CCR3 receptor. It is produced by endothelial cells stimulated with IL-4 or IL-13. Eotaxin-3 selectively targets cells expressing CCR3, including eosinophils, basophils, T cells and monocytes. Eotaxin-3 has similar activity to Eotaxin and Eotaxin-2, but the three Eotaxins share only a low degree of sequence homology. Recombinant human Eotaxin-3 is an 8.4 kDa protein containing 71 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

7138 | Eotaxin-3/CCL26 human recombinant DataSheet

Biomolecule/Target: CCL26

Synonyms: CCL26

Alternates names: C-C motif chemokine 22, Small-inducible cytokine A22, Macrophage-derived chemokine, MDC (1-69), Stimulated T-cell chemotactic protein 1, CC chemokine STCP-1, CCL22, MDC, SCYA22, ABCD-1, DC/B-CK, MGC34554, A-152E5.1.

Taglines: A CC chemokine that signals through the CCR3 receptor

NCBI Gene ID #: 6367

NCBI Gene Symbol: CCL22

Gene Source: Human

Accession #: O00626

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to chemoattract human CCR3/HEK 293 cells using a concentration range of 1.0-2.0 g/ml.

Biological activity: Determined by its ability to chemoattract human T cells using a concentration range of 10ng-100ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 8.1 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: It is recommended to reconstitute the lyophilized CCL22 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Amino acid sequence: GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE ICADPRVPWV KMILNKLSQ.