Epiandrosterone ELISA Kit - E4852

E4852 | Epiandrosterone ELISA Kit DataSheet

Epiandrosterone, or 3ß-androsterone, also known as 3ß-hydroxy-5a-androstan-17-one or 5α-androstan-3ß-ol17-one, is a steroid hormone with weak androgenic activity. It is a natural metabolite of dehydroepiandrosterone (DHEA) via the 5α-reductase enzyme. ’s Epiandrosterone ELISA kit is a competitive ELISA assay for the quantitative measurement of Epiandrosterone in urine, saliva, and fecal samples, or in extracted serum and plasma. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An Epiandrosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to Epiandrosterone to each well. After incubation the plate is washed and substrate is added. The substrate reacts with the bound Epiandrosterone -peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength.

Alternate Name :

Tag Line : A competitive ELISA kit for the quantitative measurement of Epiandrosterone in Saliva, Urine, Extracted Serum or Plasma, Fecal Extracts samples

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Extracted Serum or Plasma,Cell and tissue culture supernatants,Dried Fecal Extracts,Urine,Saliva

Species Reactivity : All

Applications : This ELISA kit is used for in vitro quantitative determination of Epiandrosterone

Features and Benefits : Detection Limit: 0.107 ng/mL,Sensitivity:0.120 ng/mL.,This ELISA kit is used for in vitro quantitative determination of Epiandrosterone.,Detection Range: 0.098 - 100 ng/mL