Epidermal Growth Factor (EGF), human recombinant | 4022

Human epidermal growth factor (EGF) is also known as HOMG4 and URG, and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk. Recombinant human EGF is a 6.2 kDa protein containing 53 amino acid residues. This recombinant human EGF has an N-terminal His-tag preceding the 53 amino acid sequence (MW = 8.5 kDa)

4022 | Epidermal Growth Factor EGF human recombinant DataSheet

Biomolecule/Target: EGF

Synonyms: Urogastrone, URG, EGF, epidermal growth factor

Alternates names: C-C motif chemokine 22, Small-inducible cytokine A22, Macrophage-derived chemokine, MDC (1-69), Stimulated T-cell chemotactic protein 1, CC chemokine STCP-1, CCL22, MDC, SCYA22, ABCD-1, DC/B-CK, MGC34554, A-152E5.1.

Taglines: Human EGF from E.Coli, AOF, 98% pure, biologically active, cell culture tested.

NCBI Gene ID #: 6367

NCBI Gene Symbol: CCL22

Gene Source: Human

Accession #: O00626

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract human T cells using a concentration range of 10ng-100ng/ml corresponding to a Specific Activity of 10,000-100,000IU/mg.

Results: 5.92-10.06 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 8.1 kDa

Concentration: N/A

Appearance: Recombinant EGF is available as a lyophilized powder

Physical form description: EGF protein is lyophilized with no additives

Reconstitution Instructions: It is recommended to reconstitute the lyophilized CCL22 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Amino acid sequence: GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE ICADPRVPWV KMILNKLSQ.