356

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit | P-3001

(No reviews yet) Write a Review
SKU:
356-P-3001-GEN
Availability:
Usually shipped in 5 working days
€1,992.00 - €2,922.00

Description

We recommend using a newer version of this product: EpiQuik DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric)

The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit is a complete set of optimized buffers and reagents that allows the experimenter to measure DNA methyltransferase activity or inhibition at extremely fast speeds. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment. The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit is suitable for measuring DNMT activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria. The kit has the following advantages:

  • Very rapid procedure, which can be completed within 3 hours.
  • Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Extremely simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit is designed for measuring total DNMT activity (de novo, maintenance). In an assay with this kit, the unique cytosine-rich DNA substrate is stably coated on the strip wells. These wells are specifically treated to have a high DNA absorption ability. DNMT enzymes transfer a methyl group to cytosine from Adomet to methylate DNA substrate. The methylated DNA can be recognized with an anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit DataSheet

View AllClose

Additional Information

Category:
*Immunoassay Kits
Research Area:
DNA Methylation
Target Applications:
Activity Measurement
Input Type:
DNA
Vessel Format:
Microplate-based
Shipping:
Blue Ice
View AllClose