EPO-alpha, human recombinant | 4763

Erythropoietin (EPO) is a glycoprotein hormone that is principally known for its role in erythropoiesis, where it is responsible for stimulating proliferation and differentiation of erythroid progenitor cells. The differentiation of CFU-E (Colony Forming Unit -Erythroid) cells into erythrocytes can only be accomplished in the presence of EPO. Physiological levels of EPO in adult mammals are maintained primarily by the kidneys, whereas levels in fetal or neonatal mammals are maintained by the liver. EPO also can exert various non-hematopoietic activities, including vascularization and proliferation of smooth muscle, neural protection during hypoxia, and stimulation of certain B cells. ’s Human EPO contains 166 amino acid residues and has a calculated molecular weight of approximately 18.4 kDa. As a result of glycosylation, Recombinant Human EPO migrates with an apparent molecular mass of 37.0 kDa by SDS-PAGE Gel, under reducing and non-reducing conditions.

4763 | EPO-alpha human recombinant new size DataSheet

Biomolecule/Target: N/A

Synonyms: Erythropoietin-, EPO-, Epoetin, EP, MGC138142

Alternates names: Erythropoietin-, EPO-, Epoetin, EP, MGC138142

Taglines: A glycoprotein hormone that controls erythropoiesis

NCBI Gene ID #: 14164

NCBI Gene Symbol: FGF-1

Gene Source: Human

Accession #: P61148

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 90%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Determined by a cell proliferation assay using TF-1 cells. The expected ED50 for this effect is 0.8-1.0 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 37 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Each mg of lyophilized protein contains 0.58 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1 to 1.0 mg/ml. This solution can then be diluted into other aqueous buffers containing a carrier protein (like 0.1% BSA) and stored in working aliquots at -20°C to -80ºC for future use.

Amino acid sequence: N/A