ESR1 Antibody | 7437

ESR1 Antibody | 7437 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Pig: (100%) , Horse: (100%)

Immunogen: ESR1 antibody was raised against an 18 amino acid peptide near the center of human ESR1.
The immunogen is located within amino acids 250 - 300 of ESR1.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: ESR1 antibody can be used for detection of ESR1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: ESR1 antibody is human, mouse and rat reactive.

Positive Control 1: Cat. No. 1463 - Rat Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 65 kDa
Observed: 62 kDa

Validation: N/A

Isoform: N/A

Purification: ESR1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ESR1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ESR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, Estrogen receptor, ER-alpha, ER

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ESR1 Antibody: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1) . Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta) , contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1, 2) . Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1) , plays a large role in regulating ER alpha activity (3) .