Estrone-3-Glucuronide (E1G) ELISA Kit - E4850

E4850 | Estrone-3-Glucuronide (E1G) ELISA Kit DataSheet

Estrone-3-glucuronide, C24H30O8 , (1,3,5(10)-estratrien-3-ol-17-one glucosiduronate, E1G) is the principle secreted form of circulating estradiol in mammals. Clinical studies have indicated the utility of measuring estrone-3-glucuronide (E1G) and pregnanediol-3aglucuronide (PDG) in samples of urine to monitor ovarian function in females. There is substantial evidence supports an association of endogenous reproductive hormone exposure with increased risk of reproductive cancers. Greater estrogen exposure, assessed via indirect indicators such as number of years spent having menstrual cycles or direct indicators such as hormone measures , is associated with increased risk for cancers of the breast and ovary. ’s E1G ELISA kit is a competitive ELISA assay for the quantitative measurement of E1G in extracted serum and plasma, or in urine, extracted dried fecal samples, and tissue culture media samples. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An E1G-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to E1G to each well. After incubation the plate is washed and substrate is added. The substrate reacts with the bound E1G-peroxidase conjugate. After a short incubation, the reaction is read at 450 nm wavelength.

Alternate Name :

Tag Line : A competitive ELISA kit for the quantitative measurement of E1G in dried fecal, urine and tissue culture samples.

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Fecal Extracts, Plasma, Urine and tissue culture samples

Species Reactivity : All

Applications : This ELISA kit is used for in vitro quantitative determination of E1G.

Features and Benefits : Detection Limit: 8.76 pg/mL,Sensitivity: 7.38 pg/mL,This ELISA kit is used for in vitro quantitative determination of E1G.,Detection Range: 1,000 to 15.625 ng/mL