Estrone ELISA kit | 55R-IB59105

Estrone ELISA kit | 55R-IB59105 |

Activity Code: ACTIVE

Product Type: Research Kits

Product Subtype: Kits

Research Area: Neuroscience

Short Description: ELISA kit for the detection of Estrone in the research laboratory

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: N/A

Residues: N/A

Tag/conjugate: N/A

Protein Type: N/A

Expression System: N/A

Grade & Purity: N/A

Methode of Purification: N/A

Source: N/A

Concentration: N/A

Form & Buffer: N/A

Storage: Store at 2-8 deg C

Shipping Information: *Blue Ice*

Applications: ELISA

Bioactivity: N/A

Usage Recommendations: Optimal conditions to be determined by end user

Assay Information: The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition  occurs between an unlabeled antigen (present in standards, control and patient samples) and an enzymelabelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The  intensity of the colour formed is inversely proportional to the concentration of estrone in the sample. A set of standards is used to plot a standard curve from which the amount of estrone in patient samples and controls can be directly read.