Ethanolamine Kinase 2, human recombinant | P1158

Ethanolamine kinase, ETNK, (E.C. 2.7.1.82), catalyzes the ATP-dependent phosphorylation of free intracellular ethanolamine and this is the first committed step of the de novo CDP-ethanolamine pathway for phosphatidylethanolamine (PE) biosynthesis in mammalian tissues. PE is a major phospholipid component of cell membranes and it is involved in diacylglyercol and G protein-dependent signal transduction. ETNK plays a critical role in cell growth and it is implicated to associate with abnormal choline and ethanolamine phospholipid metabolism identified in breast, prostate, ovarian and cervical cancers. Two isoforms of ethanolamine kinase, ETNK1 and ETNK2, have been identified in mammalian cells and they share > 50% in homology. ’s active human ETNK2 is suitable for functional assays, high-throughput screening and preclinical studies in drug discovery.

P1158 | Ethanolamine Kinase 2 human recombinant DataSheet

Biomolecule/Target: ETNK2

Synonyms: Ethanolamine Kinase 2, ETNK2, EKI2, ethanolamine phosphokinase, Ethanolamine kinase-like protein

Alternates names: Ethanolamine Kinase 2, ETNK2, EKI2, ethanolamine phosphokinase, Ethanolamine kinase-like protein

Taglines: Catalyzes the first step of phosphatidylethanolamine (PtdEtn) biosynthesis via the cytidine diphosphate

NCBI Gene ID #: 2247

NCBI Gene Symbol: FGF2

Gene Source: Human

Accession #: P09038

Recombinant: Yes

Source: E.coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to convert 1 mole of ATP to ADP per minute at pH 7 at 25°C

Biological activity: Specific activity is 2 mU/mg

Results: 2 mU/mg

Binding Capacity: N/A

Unit Definition: One unit of enzyme converts 1 mole of ATP into ADP at 25ºC and pH 7

Molecular Weight: 45 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM Tris, pH 8.0, 150 mM NaCl, 2 mM DTT, 2 mM EDTA and 20% glycerol

Reconstitution Instructions: N/A

Amino acid sequence: N/A