Exo1 | exoglucanase isoenzyme 1 | AS08 322

Exo1 | exoglucanase isoenzyme 1 | AS08 322 | Gentaur UK, US & Europe Distribution

Immunogen: synthetic peptide conjugated to KLH

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: N/A

Recommended Dilutions: 1 : 10 000 (ELISA), 1 : 5 000 (WB)

Molecular weight: 66 kDa

Confirmed Reactivity: Hordeum vulgare

Predicted Reactivity: Oryza sativa, Zea mays Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: N/A

Background: Family 3 beta-d-glucan glucohydrolases are widely distributed in higher plants. The enzymes catalyse the hydrolytic removal of beta-d-glucosyl residues from non-reducing termini of a range of beta-d-glucans and beta-d-oligoglucosides. Their broad specificity can be rationalized from X-ray crystallographic data obtained from a barley beta-d-glucan glucohydrolase in complex with non-hydrolysable S-glycoside substrate analogues, and from molecular modelling of enzyme-substrate complexes. The glucosyl residue occupying binding subsite -1 is tightly locked into a fixed position through extensive hydrogen bonding with six amino acid residues near the bottom of an active site pocket. In contrast, the glucosyl residue at subsite +1 is located between two tryptophan residues at the entrance of the pocket, where it is less tightly constrained. The relative flexibility of binding at subsite +1, coupled with the projection of the remainder of bound substrate away from the enzyme's surface, means that the overall active site can accommodate a range of substrates with variable spatial dispositions of adjacent beta-d-glucosyl residues. The broad specificity for glycosidic linkage type would enable the enzyme to perform diverse functions during plant development.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Carbohydrates

Research Area: Plant Biology