Exonuclease I | 9217

Exonuclease I hydrolyzes single-stranded DNA in the 3'5' direction, releasing 5'-mononucleotides and leaving the terminal 5'-dinucleotide intact. Hydrolysis is processive and cannot proceed if the 3' terminus is phosphorylated.

9217 | Exonuclease I DataSheet

Biomolecule/Target: N/A

Synonyms: Exo I

Alternates names: Exo I

Taglines: Hydrolyzes single-stranded DNA in the 3'5' direction, releasing 5'-mononucleotides and leaving the terminal 5'-dinucleotide intact.

NCBI Gene ID #: 2247

NCBI Gene Symbol: FGF2

Gene Source: N/A

Accession #: P09038

Recombinant: Yes

Source: E. coli strain containing an overproducing clone of E. coli Exonuclease I.

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): 10 unit/l

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit is the amount of enzyme which catalyzes the release of 10 nmol of acid-soluble nucleotide from denatured DNA in 30 min at 37°C under standard conditions.

Molecular Weight: 55.0 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Exonuclease I in 20 mM Tris-HCI (pH 7.5), 5 mM 2-mercaptoethanol, 0.5 mM EDTA, 50% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: N/A