EZAct™ Procathepsin Activation Buffer | 1030

Cathepsins (CTS) are proteases that degrade proteins. They are present in all animals and other organisms. These proteases are of three types: cysteine proteases, aspartyl protease and serine proteases. Cathepsins are produced as inactive proenzyme precursors that are subsequently activated in lysosomes. ’s EZAct™ Procathepsin Activation Buffer is a ready-to-use buffer for generation of active cysteine/aspartyl Cathepsins by auto-activation of the Procathepsins.

1030 | EZAct™ Procathepsin Activation Buffer DataSheet

Sort Name: EZAct™ Procathepsin Activation Buffer

Label Name: Procathepsin Activation Buffer

Taglines: A ready-to-use buffer for generation of active Cathepsins by auto-activation of the Procathepsins.

Product Highlights: APPLICATIONS: 1. To generate active Cathepsins from inactive Procathepsins. 2. To test the activity of Cathepsins. 3. To screen for Cathepsin Inhibitor candidates. ACTIVATION PROTOCOL: 1. Adjust the concentration of the target Procathepsin to 0.5-5 mg/ml with the appropriate buffer. 2. Add equal amounts of EZAct™ Procathepsin Activation Buffer and mix well by pipetting up and down. 3. Incubate at the appropriate temperature (4°/ 22°/ 37°C) for the study. 4. Check the cathepsin activation by testing with an appropriate substrate. Note: ’s Cathepsin Assay kits can be used for assaying the Cathepsin activity. Choose from assay kits: K140, K141, K142, K143, K144, K145 and K146.

Packaging: EA