EZH1 Antibody | 6261

EZH1 Antibody | 6261 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: EZH1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EZH1.
The immunogen is located within amino acids 180 - 230 of EZH1.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: EZH1 antibody can be used for detection of EZH1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Multiple isoforms of EZH1 are known to exist. EZH1 antibody is predicted to not cross-react with EZH2

Positive Control 1: Cat. No. 1402 - Mouse Lung Tissue Lysate

Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: EZH1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: EZH1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: EZH1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: EZH1 Antibody: KMT6B, KIAA0388, Histone-lysine N-methyltransferase EZH1, ENX-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: EZH1 Antibody: EZH1 was initially identified as a homolog of the drosophila Enhancer of Zeste through transcription mapping of chromosome 17q21. Both EZH1 and the related protein EZH2 can form complexes with the noncanonical Polycomb repressive complex-2 (PRC2) and maintain repressive chromatin, but the PRC2-EZH1 complex mediates methylation of histone H3. Both EZH1 and EZH2 are thought to function in the maintenance of embryonic stem cell pluripotency and plasticity and recently have been shown to be essential for hair follicle homeostasis and wound repair.