EZH2 Antibody | 6263

EZH2 Antibody | 6263 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: EZH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human EZH2.
The immunogen is located within amino acids 190 - 240 of EZH2.

Research Area: Stem Cell

Tested Application: E, WB, ICC, IF

Application: EZH2 antibody can be used for detection of EZH2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Multiple isoforms of EZH2 are known to exist. EZH2 antibody is predicted to not cross-react with EZH1.

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: Cat. No. 17-010 - HEK293 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: EZH2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: EZH2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: EZH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: EZH2 Antibody: WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A, Histone-lysine N-methyltransferase EZH2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: EZH2 Antibody: EZH2 was initially identified as a homolog of the drosophila Enhancer of Zeste through exon trap screening of chromosome 21. Both EZH2 and the related protein EZH1 can form complexes with the noncanonical Polycomb repressive complex-2 (PRC2) and maintain repressive chromatin, but the PRC2-EZH1 complex mediates methylation of histone H3. Both EZH1 and EZH2 are thought to function in the maintenance of embryonic stem cell pluripotency and plasticity and recently have been shown to be essential for hair follicle homeostasis and wound repair. Overexpression of EZH2 has been reported as a marker for advanced and metastatic cancers.