F1A alpha Antibody | 2279

F1A alpha Antibody | 2279 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Chicken: (79%)

Immunogen: F1A antibody was raised against a 14 amino acid peptide near the carboxy terminus of human F1A.
The immunogen is located within the last 50 amino acids of F1A alpha.

Research Area: Apoptosis

Tested Application: E, WB, IHC-P, IF

Application: F1Ab antibody can be used for detection of F1Ab by Western blot at 1 μg/mL. An approximately 70 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1404 - Mouse Liver Tissue Lysate

Positive Control 2: Cat. No. 1464 - Rat Liver Tissue Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 70 kDa

Validation: N/A

Isoform: N/A

Purification: F1A alpha Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: F1A alpha Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: F1A alpha antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: F1A alpha Antibody: F1AA, F1A-ALPHA, FEM1-beta, F1AA, KIAA0396, Protein fem-1 homolog B, FEM1b

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: F1A alpha Antibody: Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aalpha and FEM1β. F1Aalpha/FEM1β is the homologue of C. elegans sex determining protein FEM-1. FEM-1/F1Aalpha is cleaved by CED-3 and caspase. FEM-1/F1Aalpha associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aalpha induces apoptosis. F1Aalpha is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aalpha is expressed in a variety of human and mouse tissues.