FANCM Antibody (C-Term) [APR33374G]

FANCM Antibody (C-Term) [APR33374G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This FANCM antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1752-1783 amino acids from human FANCM.

Cellular Localisation: Nucleus

Molecular Weight: 232191

Clone: Polyclonal

Gene Name: FANCM

Gene ID: 57697

Function: DNA-dependent ATPase component of the Fanconi anemia (FA) core complex (PubMed:16116422) . Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross- linking drugs, and prevention of chromosomal breakage (PubMed:16116422, PubMed:19423727, PubMed:20347428, PubMed:20347429, PubMed:29231814) . In complex with CENPS and CENPX, binds double-stranded DNA (dsDNA), fork- structured DNA (fsDNA) and Holliday junction substrates (PubMed:20347428, PubMed:20347429) . Its ATP-dependent DNA branch migration activity can process branched DNA structures such as a movable replication fork. This activity is strongly stimulated in the presence of CENPS and CENPX (PubMed:20347429) . In complex with FAAP24, efficiently binds to single-strand DNA (ssDNA), splayed-arm DNA, and 3'-flap substrates (PubMed:17289582) . In vitro, on its own, strongly binds ssDNA oligomers and weakly fsDNA, but does not bind to dsDNA (PubMed:16116434) .

Summary: Tissue Location: Expressed in germ cells of fetal and adult ovaries. In fetal ovaries, it is present in oogonia but expression is stronger in pachytene stage oocytes. Expressed in oocytes arrested at the diplotene stage of prophase I during the last trimester of pregnancy and in adults (PubMed:29231814) . Expressed in the testis (PubMed:30075111) .

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: Fanconi anemia group M protein, Protein FACM, ATP-dependent RNA helicase FANCM, Fanconi anemia-associated polypeptide of 250 kDa, FAAP250, Protein Hef ortholog, FANCM, KIAA1596