Fat Free Antibody | 6979

Fat Free Antibody | 6979 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Chicken: (82%)

Immunogen: Fat Free antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Fat Free.
The immunogen is located within amino acids 620 - 670 of Fat Free.

Research Area: Homeostasis

Tested Application: E, WB

Application: Fat Free antibody can be used for detection of Fat Free by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Specificiy: At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms.

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 86 kDa

Validation: N/A

Isoform: N/A

Purification: Fat Free Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Fat Free Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Fat Free antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Fat Free Antibody: FFR, ANG2, ANG3, C11orf2, C11orf3, FFR, PP5382, Vacuolar protein sorting-associated protein 51 homolog, Another new gene 2 protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Fat Free Antibody: Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN) . Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.