FBP2, human recombinant | 7804

FBP2 belongs to the FBPase class 1 family. The protein is a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Recombinant human FBP2 protein, fused to His-tag at N-terminus, was expressed in E.Coli and purified by using conventional chromatography techniques.

7804 | FBP2 human recombinant DataSheet

Biomolecule/Target: FBP2

Synonyms: Fructose-1, 6-bisphosphatase isozyme 2.

Alternates names: Fructose-1, 6-bisphosphatase isozyme 2.

Taglines: A gluconeogenesis regulatory enzyme

NCBI Gene ID #: 54250

NCBI Gene Symbol: FGF-2

Gene Source: Human

Accession #: P13109

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 0.16 unit/ml, in which one unit will convert 1.0 µmole of fructose 1, 6-diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 25ºC

Results: Specific activity is > 0.16 unit/ml

Binding Capacity: N/A

Unit Definition: One unit will convert 1.0 µmole of fructose 1, 6-diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 25ºC

Molecular Weight: 39.0 kDa (362 aa, 1-339 aa + His Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution 20 mM Tris-HCl buffer, pH 8.0, 10% glycerol, 0.15 M NaCl and 1 mM DTT.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSMTDRSPF ETDMLTLTRY VMEKGRQAKG TGelTQLLNS MLTAIKAISS AVRKAGLAHL YGIAGSVNVT GDEVKKLDVL SNSLVINMVQ SSYSTCVLVS EENKDAIITA KEKRGKYVVC FDPLDGSSNI DCLASIGTIF AIYRKTSEDE PSEKDALQCG RNIVAAGYAL YGSATLVALS TGQGVDLFML DPALGEFVLV EKDVKIKKKG KIYSLNEGYA KYFDAATTEY VQKKKFPEDG SAPYGARYVG SMVADVHRTL VYGGIFLYPA NQKSPKGKLR LLYECNPVAY IIEQAGGLAT TGTQPVLDVK PEAIHQRVPL ILGSPEDVQE YLTCVQKNQA GS