FGF-16, human recombinant | 7149

FGF-16 is a heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-16 signals through FGFR 2c and 3c. FGF-16 plays a role in the development of the central nervous system. Recombinant human FGF-16 is a 23.6 kDa protein consisting of 206 amino acid residues.

7149 | FGF-16 human recombinant DataSheet

Biomolecule/Target: FGF-16

Synonyms: Fibroblast Growth Factor-16, FGFG

Alternates names: Vascular Endothelial Growth Factor-B, VEGFB, VEGF-related factor

Taglines: Promotes cellular proliferation and differentiation

NCBI Gene ID #: 7423

NCBI Gene Symbol: VEGFB

Gene Source: Human

Accession #: P49765

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by the dose dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors. The expected ED50 is 0.5 ng/ml corresponding to a specific activity of 2 x 10^6 units/mg.

Biological activity: Per researcher’s feedback, the protein is biologically active as determined by the dose-dependent stimulation of the proliferation of human mbilical vein endothelial cells (HUVEC) in the presence of human VEGF165. The expected ED for this effect is 0.5-5.0 g/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.63 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Tris, pH 9.0, 0.1 M L-Arginine and 0.4 mM DTT.

Reconstitution Instructions: Reconstitute in 0.1% acetic acid to a concentration of 0.1-1.0 mg/ml. The solution should be stored at -70°C.

Amino acid sequence: N/A