FGF-2/FGF-basic, human recombinant | 4037

FGF-2 (FGF2, FGF 2, FGF-basic, basic FGF, bFGF) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-2 protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different FGF-2 isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

4037 | FGF-2/FGF-basic human recombinant DataSheet

Biomolecule/Target: FGF-2

Synonyms: Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b, Fibroblast Growth Factor

Alternates names: Galactein 7, Galectin7, Gal-7, HKL-14, PI7, p53-induced gene 1 protein

Taglines: Recombinant FGF2 from E.Coli, animal origin free, 95% pure, biologically active, cell culture tested. A growth factor involved in angiogenesis, wound healing and embryonic development.

NCBI Gene ID #: 3963

NCBI Gene Symbol: LGALS7

Gene Source: Human

Accession #: P47929

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 95%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to agglutinate human red blood cells. The ED for the effect is typically 1-7 µg/ml.

Results: <0.5 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 15.4 kDa

Concentration: N/A

Appearance: Recombinant FGF-2 is available as a lyophilized powder

Physical form description: FGF-2 protein is lyophilized from a 1 mg/ml solution of 5 mM Tris pH 7.6 + 150 mM NaCl

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A