Description
FGF-acidic Antibody | XP-5136 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-acidic (human Fibroblast Growth Factor-acidic) .
Research Area: Growth Factors, Antibody Pairs, Functional Assays
Tested Application: E, WB, Neut
Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hFGF-acidic (30.0 ng/mL) , a concentration of 0.46 - 0.9 μg/mL of this antibody is required.
ELISA:
To detect hFGF-acidic by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-acidic.
Sandwich: To detect hFGF-acidic by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human FGF-acidic (XP-5136Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-acidic.
Western Blot:
To detect hFGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Specificiy: N/A
Positive Control 1: N/A
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Anti-hFGF-acidic specific antibody was purified by affinity chromatography employing immobilized hFGF-acidic matrix.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Lyophilized
Buffer: N/A
Concentration: N/A
Storage Condition: FGF-acidic antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Alternate Name: AFGF, ECGF, FGFA, ECGFA, ECGFB, FGF-1, HBGF1, HBGF-1, GLIO703, ECGF-beta, FGF-alpha, Fibroblast growth factor 1, Acidic fibroblast growth factor
User Note: Centrifuge vial prior to opening.
BACKGROUND: N/A