FGF-acidic, Bovine Brain | P1589

Acidic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

P1589 | FGF-acidic, Bovine Brain DataSheet

Biomolecule/Target :

Synonyms: HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a, Fibroblast Growth Factor Acidic

Alternates names: HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a, Fibroblast Growth Factor Acidic

Taglines: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration.

NCBI Gene ID #: 281160

NCBI Gene Symbol: FGF1

Gene Source: Bovine

Accession #: P03968

Recombinant: FALSE

Source: Bovine brain

Purity by SDS-PAGE: >90%

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): Stimulates growth of bovine capillary endothelial cells by 3-5 fold over 5% calf serum at 10-25ng/ml FGF.

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: FGF-1 contains a 17 kDa and a 20 kDa polypeptide chain. The 17 kDa peptide is derived from the 20 K peptide by restricted proteolysis.

Concentration:

Appearance: Lyophilized

Physical form description: Lyophilized from 0.5 ml solution containing 1 mM sodium phosphate, pH 7 after filtration over a low binding membrane.

Reconstitution Instructions: Reconstitute the lyophilized aFGF in sterile 50 mM Na2HPO4 pH-7, and 0.5% albumin. The Recommended concentration in cell culture: 1-20 ng/ml.

Amino acid sequence:

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans