FGF basic protein | 30R-AF015

FGF basic protein | 30R-AF015 |

Activity Code: ACTIVE

Product Type: Proteins

Product Subtype: Recombinant Proteins

Research Area: Cancer

Short Description: Purified recombinant Human FGF basic protein

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: Human

Residues: Fibroblast Growth Factor protein containing 155 amino acids.

Tag/conjugate: N/A

Protein Type: Recombinant

Expression System: E.coli

Grade & Purity: > 98% pure

Methode of Purification: FGF protein (Basic)

Source: N/A

Concentration: N/A

Form & Buffer: Sterile Filtered Lyophilized from a concentrated solution containing PBS pH 7.4. Reconstitute in distilled water to at least 100 ug/ml.  Please Note:  Buffer may change with new Lots.

Storage: Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping Information: *Blue Ice*

Applications: User optimized

Bioactivity: The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is

Usage Recommendations: N/A

Assay Information: Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard.