Fish 5-HT (5-Hydroxytryptamine) ELISA Kit | ELK9416

Fish 5-HT (5-Hydroxytryptamine) ELISA Kit | ELK9416 | Gentaur UK, US & Europe Distribution

Alternative Names: 5HT; Serotonin; N-Methyl-Gamma

Species: Fish

Assay Type: Competitive Inhibition

Sensitivity: 3.97 ng/mL

Standard: 900 ng/mL

Detection Range: 14.07-900 ng/mL

Sample Type: serum and plasma

Assay Lenght: 2h

Research Area: Signal transduction;Infection immunity;Neuro science;Gastroenterology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxytryptamine (5-HT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptamine (5-HT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine (5-HT) in the samples is then determined by comparing the OD of the samples to the standard curve.