FKBP6 protein (His tag) | 80R-1324

FKBP6 protein (His tag) | 80R-1324 |

Activity Code: ACTIVE

Product Type: Proteins

Product Subtype: Conjugated Proteins

Research Area: Immunology

Short Description: Purified recombinant Human FKBP6 protein

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: Human

Residues: 1-327 amino acids: MGSSHHHHHH SSGLVPRGSH MGGSALNQGV LEGDDAPGQS LYERLSQRML DISGDRGVLK DVIREGAGDL VAPDASVLVK YSGYLEHMDR PFDSNYFRKT PRLMKLGEDI TLWGMELGLL SMRRGELARF LFKPNYAYGT LGCPPLIPPN TTVLFEIELL DFLDCAESDK FCALSAEQQD QFPLQKVLKV AATEREFGNY LFRQNRFYDA KVRYKRALLL LRRRSAPPEE QHLVEAAKLP VLLNLSFTYL KLDRPTIALC YGEQALIIDQ KNAKALFRCG QACLLLTEYQ KARDFLVRAQ KEQPFNHDIN NELKKLASCY RDYVDKEKEM WHRMFAPCGD GSTAGES

Tag/conjugate: His tag

Protein Type: Recombinant

Expression System: E.coli

Grade & Purity: > 95% pure

Methode of Purification: N/A

Source: N/A

Concentration: 0.5 mg/ml

Form & Buffer: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 40% glycerol, 1mM EDTA and 50mM NaCl.

Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping Information: *Blue Ice*

Applications: SDS-PAGE

Bioactivity: Specific activity is > 50 nM/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1C in Tris-Hcl pH8.0 using chymotrypsin.

Usage Recommendations: N/A

Assay Information: 1. Prepare assay buffer into a suitable container and pre-chilling on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA) . 2. Add recombinant FKBP6 protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes.