FLI1 Antibody | 7449

FLI1 Antibody | 7449 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Immunogen: FLI1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human FLI1.
The immunogen is located within the last 50 amino acids of FLI1 .

Research Area: Stem Cell

Tested Application: E, WB, IF

Application: FLI1 antibody can be used for detection of FLI1 by Western blot at 0.5 - 1 μg/ml.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: FLI1 antibody is human, mouse and rat reactive. At least four isoforms of FLI1 are known to exist; this antibody will detect all four.

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: Cat. No. 17-001 - HeLa Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 50 kDa
Observed: 52kDa

Validation: N/A

Isoform: N/A

Purification: FLI1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: FLI1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: FLI1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: FLI1 Antibody: EWSR2, SIC-1, BDPLT21

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The Friend leukemia virus integration 1 (FLI1) protein is a transcription factor containing an ETS DNA-binding domain and is highly expressed in hematopoietic cell lineages and vascular endothelial cells (1) . FLI1 plays important roles in megakaryocytic differentiation (2) and vascular homeostasis (3) . The FLI1 gene can undergo a t (11;22) (q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. (4) . Abnormal expression of FLI1 can be used as an adverse prognostic indicator for acute myeloid leukemia (5) .