Description
FLI1 Antibody | 7449 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen: FLI1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human FLI1.
The immunogen is located within the last 50 amino acids of FLI1 .
Research Area: Stem Cell
Tested Application: E, WB, IF
Application: FLI1 antibody can be used for detection of FLI1 by Western blot at 0.5 - 1 μg/ml.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: FLI1 antibody is human, mouse and rat reactive. At least four isoforms of FLI1 are known to exist; this antibody will detect all four.
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: Cat. No. 17-001 - HeLa Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 50 kDa
Observed: 52kDa
Validation: N/A
Isoform: N/A
Purification: FLI1 Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: FLI1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: FLI1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: FLI1 Antibody: EWSR2, SIC-1, BDPLT21
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The Friend leukemia virus integration 1 (FLI1) protein is a transcription factor containing an ETS DNA-binding domain and is highly expressed in hematopoietic cell lineages and vascular endothelial cells (1) . FLI1 plays important roles in megakaryocytic differentiation (2) and vascular homeostasis (3) . The FLI1 gene can undergo a t (11;22) (q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. (4) . Abnormal expression of FLI1 can be used as an adverse prognostic indicator for acute myeloid leukemia (5) .