FLIP Antibody | 1156

FLIP Antibody | 1156 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Mouse

Homology: Predicted species reactivity based on immunogen sequence: Human: (77%)

Immunogen: FLIP antibody was raised against a 17 amino acid peptide near the carboxy terminus of mouse FLIP.
The immunogen is located within the last 50 amino acids of FLIP.

Research Area: Apoptosis

Tested Application: E, WB, ICC

Application: FLIP antibody can be used for the detection of FLIP by Western blot at 1-2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.

Specificiy: FLIP has short form (FLIPS) and long form (FLIPL) and antibody recognizes the FLIPL only.

Positive Control 1: Cat. No. 1204 - K562 Cell Lysate

Positive Control 2: Cat. No. 1212 - 3T3 Cell Lysate

Positive Control 3: Cat. No. 17-201 - 3T3/BALB Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 53 kDa
Observed: 58 kDa

Validation: N/A

Isoform: N/A

Purification: FLIP Antibody is Antibody is DEAE purified.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: FLIP Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: FLIP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, CASH, CASP8 and FADD-like apoptosis regulator, Caspase homolog

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED-3 protease family.Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP respectively. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and MRIT. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1.