flt3-Ligand Antibody (biotin) | XP-5138Bt

flt3-Ligand Antibody (biotin) | XP-5138Bt | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFlt3-Ligand.

Research Area: Signal Transduction, Antibody Pairs, Functional Assays

Tested Application: E, WB, Neut

Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/mL) , a concentration of 4.0-6.0 ng/mL of this antibody is required.ELISA:
Sandwich:
To detect hFlt-3 Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Flt-3 Ligand (XP-5138Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFlt-3 Ligand.
Western Blot:
To detect hFlt3-Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFlt3-Ligand is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Anti-hFlt3-Ligand specific antibody was purified by affinity chromatography and then biotinylated.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Biotin

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: flt3-Ligand antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: FL, FLT3LFms-related tyrosine kinase 3 ligand, SL cytokine, Flt3 ligand

User Note: Centrifuge vial prior to opening.

BACKGROUND: Flt3-Ligand is a growth factor important for the proliferation of hematopoietic cells. Flt3-Ligand binds to, and transmits signals through, the receptor tyrosine kinase known as FMS-like Tyrosine Kinase-3 (FLT-3) . Flt3-Ligand promotes long-term expansion and differentiation of human pro-B-cells in the presence of IL-7 or in combination of IL-7 and IL-3. Human Flt3-Ligand can stimulate the proliferation of cells expressing murine FLT-3 receptors.