Follistatin, human recombinant | 4708

Follistatin belongs to a group of structurally-diverse diffusible proteins that binds to TGF- ligands and inhibit their activity by hindering access to signaling receptors. This naturally occurring antagonist binds to Activin, BMP-2,-4,-6,-7, Myostatin, GDF-11, and TGF-1. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues. Recombinant human Follistatin is a 31.5 kDa protein containing 288 amino acids including 36 cysteine residues.

4708 | Follistatin human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: FST, FS, Activin-binding protein

Alternates names: NOG

Taglines: An autocrine glycoprotein involved in binding and bioneutralization of members of the TGF- superfamily

NCBI Gene ID #: 9241

NCBI Gene Symbol: NOG

Gene Source: Human

Accession #: Q13253

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to inhibit 5 ng/ml of BMP-4-induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED for the effect is 0.05-0.08 µg/ml of Noggin

Results: 0.1-0.4 µg/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 23.1 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 75 mM NaCl

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1 to 1.0 mg/ml. Note: Due to solubility reasons the protein should be kept at low pH. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A