FOX1 | plasma membrane ferroxidase FLP1 | AS06 120

FOX1 | plasma membrane ferroxidase FLP1 | AS06 120 | Gentaur UK, US & Europe Distribution

Immunogen: residues 394-646 from Chlamydomonas reinhardtii FOX1 A8IZT9 fused to TrxA

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: immunofluorescence (IF), Western blot (WB)

Related Products: AS07 260 | Anti-H+ATPase, plasma membrane rabbit marker antibodiesCollection of antibodies to Chlamydomonas proteinsAlgal protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 1000-1 : 5000 (WB)

Molecular weight: 122 | 120 kDa

Confirmed Reactivity: Chlamydomonas reinhardtii

Predicted Reactivity: Chlamydomonas reinhardtii

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.

Background: A copper-containing protein in Chlamydomonas reinhardtii is a plasma membrane ferroxidase, encoded by the FOX1 gene, involved in Fe assimilation. FOX1 is most highly related to the mammalian homologues hephaestin and ceruloplasmin; its occurrence and pattern of expression in Chlamydomonas indicate a role for copper in iron assimilation in a photosynthetic species.

Reconstitution: For reconstitution add 200 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Ion metabolism

Research Area: Plant Biology, Photosynthesis