Frizzled 2 Antibody | 46-844

Frizzled 2 Antibody | 46-844 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat

Immunogen: The immunogen for this antibody is: RYATLEHPFHCPR

Research Area: Signal Transduction, Stem Cell, Cancer

Tested Application: E, WB, IF, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60-65kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24kDa was also observed in some lysates which was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5ug/ml. Paraffin embedded Human Liver. Recommended concentration: 2.5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10ug/ml.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: FZD2, frizzled homolog 2 (Drosophila) , frizzled 2

User Note: N/A

BACKGROUND: N/A