FtsH1-11 | ATP-dependent zinc metalloprotease FtsH1-11 | AS11 1789

FtsH1-11 | ATP-dependent zinc metalloprotease FtsH1-11 | AS11 1789 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide dereived from sequences of all known FtsH isoforms of Arabidopsis thaliana: FtsH1 Q39102 (At1g50250), FtsH2 O80860 (At2g30950), FtsH3 Q84WU8 (At2g29080), FtsH4 O80983 (At2g26140), FtsH5 Q9FH02 (At5g42270), FtsH6 Q1PDW5 (At5g15250), FtsH7 Q9SD67 (At3g47060), FtsH8 Q8W585 (At1g06430), FtsH9 Q9FIM2 (At5g58870), FtsH10 Q8VZI8 (At1g07510), FtsH11 Q9FGM0 (At5g53170) as well as 4 FtsH isoforms of Synechocystis sp. PCC6803NP_440330.1, NP_442160.1, NP_440797.1, NP_440525.1

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Blue Native PAGE (BN-PAGE), Western blot (WB)

Related Products: AS11 1789S | FtsH2 positive control/quantitation standard to be used for quantification of FtsH2 AS16 3930 | anti-FtsH1 + FtsH5 | ATP-dependent zinc metalloprotease FtsH1 + FtsH5 (chloroplastic) AS16 3929 | anti-FtsH2 + FtsH8 | ATP-dependent zinc metalloprotease FtsH2 + FtsH8 (chloroplastic) AS07 204 | anti-FtsH3 + FtsH10 | ATP-dependent zinc metalloprotease FtsH3 + FtsH10 (mitochondrial) AS07 205 | anti-FtsH4 | ATP-dependent zinc metalloprotease FtsH4 (mitochondrial) AS05 094A | anti-FtsH6 | ATP-dependent zinc metalloprotease FtsH6 (chloroplastic) AS06 130 | anti-FtsH9 | ATP-dependent zinc metalloprotease FtsH9 (chloroplastic) AS07 251 | anti-FtsH10 | ATP-dependent zinc metalloprotease FtsH10 (mitochondrial) Antibodies to other proteins involved in photosynthesisPlant protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 500-1 : 2000 (WB)

Molecular weight: A. thaliana: FtsH1:76.7, FtsH2: 74, FtsH5: 75.2, FtsH6: 74.5, FtsH7: 87.8, FtsH8: 73, FtsH9:87.8 kDa (chloroplastic) ; FtsH3: 89.3, FtsH4: 77.2, FtsH10: 89.5 kDa, FtsH11:88.7 kDa (mitochondrial)

Confirmed Reactivity: Acetabularia acetabulum, Arabidopsis thaliana, Bryopsis hypnoides (ulvophytes), Guillardia theta, Hordeum vulgare, Synechocystis sp., Thalassiosira pseudonana, Thalassiosira punctigera, Zea mays

Predicted Reactivity: Diatoms including Phaeodactylum tricornutum, dicots, Chlamydomonas reinhardtii, Monocots; Nannochloropsis gaditana Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: For detection on a diatom samples, load of 5-10 µg/well is required as well as usage of enchanced chemiluminescence.The antibody also detects the chloroplast-encoded FtsH from Thalassiosira pseudonana (diatom) along with 3 related isoforms encode in the nuclear genome of Thalassiosira pseudonana.For detection in BlueNative, recommened dilution is 1: 500.

Background: FtsH belong to a family of ATP dependent peptidases. Localized in a chloroplast are following isoforms: FTSH1 (synonymes AAA, FTSH, FTSH Protease 1), Ftsh2 (VAR2, VARIEGATED 2), FtsH5 (VAR1, VARIEGATED 1), FtsH6 (FTSH PROTEASE 6), FtsH7, FtsH8. FtsH9.Localized in mitochondria are following isoforms: FtsH3 (FTSH Protease 3), FtsH4, FtsH10, FtsH11.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT1G50250, AT2G29080

Category: Global Antibodies

Research Area: Plant Biology