Fucose | AS07 268

Fucose | AS07 268 | Gentaur UK, US & Europe Distribution

Immunogen: Core fucose residues bound to the N-glycan in alpha 1, 3

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: ELISA (ELISA), Immunolocalization (IL), Western blot (WB)

Related Products: AS07 267 | Anti-xylose, rabbit antibodiesPlant and algal protein extraction buffer

Recommended Dilutions: 0, 5 µg/ml (ELISA), 1 : 40 (IL), 1 ug/10 ml (WB)

Molecular weight: 10 - 100 for various glycoproteins

Confirmed Reactivity: Higher plants, Pheodactylum tricornutum (diatom)

Predicted Reactivity: Higher plants, Phaeodactylum tricornutum

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Controls:PLA2 (phospholipase 2 from bee venom) which contains only α1.3 fucose, Sigma, product number P9279.Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250.The antibody does not recognize alpha 1, 6-fucose.

Background: This antibody specifically cross-reacts against fucose residues bound to the protein N-glycans in alpha 1, 3. This residue is characteristic of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: Carbohydrates

Research Area: Plant Biology