Description
G6PD protein | 30R-1263 |
Activity Code: ACTIVE
Product Type: Proteins
Product Subtype: Recombinant Proteins
Research Area: Proteases, Inhibitors, & Enzymes
Short Description: Purified recombinant E.coli G6PD protein
Immunogen: N/A
Host: N/A
Specificity: N/A
Cross Reactivity: N/A
Isotype: N/A
IClone: N/A
Species: E.coli
Residues: 1-491 amino acids: MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
Tag/conjugate: N/A
Protein Type: Recombinant
Expression System: E.coli
Grade & Purity: > 90% pure
Methode of Purification: N/A
Source: N/A
Concentration: 1 mg/ml
Form & Buffer: 50mM MES 6.0, 0.1mM PMSF, 2mM EDTA, 0.5mM DTT, and 10% glycerol.
Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping Information: *Blue Ice*
Applications: SDS-PAGE
Bioactivity: Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25 DEG C.
Usage Recommendations: N/A
Assay Information: 1. Prepare a 3ml reaction cocktail into a suitable container: The final concentrations are 50mM gylcylglycine, 2mM D-glucose 6-phosphate, 0.67mM beta-NADP, 10mM MgCl2. 2. Equilibrate to 25 DEG C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 4ug of recombinant G6PD into reaction cocktail and mix immediately. 4. Record the increase at A340nm for 5 minutes.