GABBR1 Antibody (N-Term) [APG03311G]

GABBR1 Antibody (N-Term) [APG03311G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This GABBR1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 18-52 amino acids from human GABBR1.

Cellular Localisation: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane {ECO:0000250|UniProtKB:Q9Z0U4}; Multi-pass membrane protein. Cell projection, dendrite {ECO:0000250|UniProtKB:Q9Z0U4}. Note=Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity) . Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane (PubMed:15617512) . {ECO:0000250|UniProtKB:Q9Z0U4, ECO:0000269|PubMed:15617512}

Molecular Weight: 108320

Clone: Polyclonal

Gene Name: GABBR1

Gene ID: 2550

Function: Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:9872316, PubMed:9872744, PubMed:15617512, PubMed:18165688, PubMed:22660477, PubMed:24305054) . Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:18165688) . Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10906333, PubMed:10773016, PubMed:10075644, PubMed:9872744, PubMed:24305054) . Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644) . Calcium is required for high affinity binding to GABA (By similarity) . Plays a critical role in the fine- tuning of inhibitory synaptic transmission (PubMed:9844003) . Pre- synaptic GABA receptor inhibits neurotransmitter release by down- regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:9844003, PubMed:9872316, PubMed:10075644, PubMed:9872744, PubMed:22660477) . Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable) . Activated by (-) -baclofen, cgp27492 and blocked by phaclofen (PubMed:9844003, PubMed:9872316, PubMed:24305054) .

Summary: Tissue Location: Highly expressed in brain (PubMed:9844003, PubMed:9753614, PubMed:9872744) . Weakly expressed in heart, small intestine and uterus. Isoform 1A: Mainly expressed in granular cell and molecular layer (PubMed:9844003) . Isoform 1B: Mainly expressed in Purkinje cells (PubMed:9844003) . Isoform 1E: Predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland (PubMed:10906333)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: Gamma-aminobutyric acid type B receptor subunit 1, GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1, GABBR1, GPRC3A