GAL-9 (Human) ELISA Kit - E5012

E5012 | GAL-9 (Human) ELISA Kit DataSheet

Galectin-9 (GAL-9) is a 36 kDa β-galactoside lectin protein that belongs to the galectin family of proteins. It is ubiquitously expressed in tissues and cells; however, it is abundantly present in immune and epithelial cells of the GI tract. Galectin-9 is involved in the negative selection of T-cells. It also induces apoptosis of CD4+ Th1 cells and CD8+ cytotoxic cells and promotes differentiation of naïve T-cells into Treg. Studies have demonstrated that Galectin-9 expression correlates with tumor cell adhesion and aggregation in melanoma, breast cancer, hepatocellular, and oral squamous cell carcinoma. Therefore, Galectin-9 could serve as an efficient target for anti-cancer therapies. ’s Galectin-9 (Human) ELISA kit quantitatively measures Galectin-9 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of Galectin-9 in the sample or standard.

Alternate Name : HUAT, LGALS9A, LGALS9

Tag Line : A Sandwich ELISA kit for quantitative measurement of Galectin-9 in human serum, plasma, tissue lysates, and other biological fluids

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Tissue lysate Other biological fluids Serum Plasma

Species Reactivity : Human

Applications : Sandwich ELISA kit for quantitative measurement of GAL-9 in serum, plasma, cell and tissue lysates, and other biological fluids

Features and Benefits : This Sandwich ELISA is highly sensitive and highly specific for the detection of GAL-9 in human samples. There is no significant cross-reactivity or interference between GAL-9 and analogues Recovery range: 85 - 105% for normal human serum and plasma samples Assay Precision: Intra-Assay CV and Inter-Assay CV < 10% Detection range: 0.156 – 10 ng/ml Sensitivity: 0.094 ng/ml