GAPDH, Active, human recombinant | P1100

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein was expressed in E.coli and purified by using conventional chromatography techniques.

P1100 | GAPDH Active human recombinant DataSheet

Biomolecule/Target: GAPDH

Synonyms: Glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD

Alternates names: Mammotropin, Luterotropic hormone, Lutetropin, Prlr, PRL, luteotropic hormone, LTH, PRLR, PRL-R, PRL R

Taglines: Involved in glycolysis

NCBI Gene ID #: 24683

NCBI Gene Symbol: PRL

Gene Source: Human

Accession #: P01237

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C

Biological activity: The ED determined by the dose-dependent stimulation of the proliferation of rat lymphoma, Nb2-11 cells was found to be in the range of 0.1-1.0 ng/ml.

Results: > 90 units/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.

Molecular Weight: 22.5 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 1 mM EDTA, 20% glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A