GAPDH Antibody | 45-060

GAPDH Antibody | 45-060 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse, Rat

Homology: Expected Species Reactivity based on sequence homology: Dog, Pig

Immunogen: The immunogen for this antibody is: C-HQVVSSDFNSDT

Research Area: Homeostasis

Tested Application: E, WB, IF, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37kDa band observed in lysates of cell lines Hek293 and HeLa and in Human Liver, Tonsil, Mouse Spleen, and Rat Brain and Spleen lysates (calculated MW of 36.1kDa according to Human NP_002037.2 and 35.8kDa according to Mouse NP_032110.1 and Rat NP_058704.1) . Recommended concentration: 0.01-0.5ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 2.5ug/ml.Immunofluorescence: Expression of the protein seen in the cytoplasm and vesicles of HeLa cells. Recommended concentration: 10ug/ml.

Specificiy: GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.

Positive Control 1: Cat. No. 1210 - 293 Cell Lysate

Positive Control 2: Cat. No. 1315 - Human Tonsil Tissue Lysate

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase

User Note: N/A

BACKGROUND: N/A