Description
GAPDH Antibody | 45-061 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human, Mouse, Rat
Homology: Expected Species Reactivity based on sequence homology: Dog
Immunogen: The immunogen for this antibody is: C-GVNHEKYDNSLK
Research Area: Homeostasis
Tested Application: E, WB, IHC, IF
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1) . Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human: PMID: 29953622 and PMID: 29158223.Immunohistochemistry:In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10ug/ml.
Specificiy: This antibody is expected to recognize both reported isoforms (NP_002037.2; NP_001243728.1) . Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.
Positive Control 1: Cat. No. 1315 - Human Tonsil Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase
User Note: N/A
BACKGROUND: N/A