GAPDH, human recombinant | 7602

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a catalytic enzyme normally known to play a role in glycolysis. GAPDH exists as a tetramer composed of 36-kDa subunits and has a range of intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, GAPDH has function in intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. GAPDH catalyzes a vital energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Recombinant human GAPDH produced in E.Coli is a single, non-glycosylated polypeptide chain containing 335 amino acids and having a molecular mass of 36 kDa. The GAPDH is purified by proprietary chromatographic techniques.

7602 | GAPDH human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: GAPDH, Glyceraldehyde 3-phosphate dehydrogenase

Alternates names: Pancreatic trypsin inhibitor, Basic protease inhibitor, BPI, BPTI, Aprotinin, AP

Taglines: Involved in glycolysis

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Human

Accession #:

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: 5 x 10 IU/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 6.5 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: 1 mg/ml solution containing 20 mM Tris pH-8, 1 mM EDTA, 1 mM DTT, and 20% glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 1 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use. For long-term storage, it is recommend to add a carrier protein (e.g., 0.1% BSA). Prevent freeze/thaw cycles.

Amino acid sequence: N/A