GAPDH Polyclonal Antibody | E-AB-40337

GAPDH Polyclonal Antibody | E-AB-40337 | Gentaur UK, US & Europe Distribution

Type: Control Antibody

Synonyms: aging associated gene 9 protein, BARS-38, cb609, G3PDH, GAPD, KNC-NDS6, OCAS, p38 component

Application: WB, IHC

Reactivity: Human, Mouse, Rat

Host: Rabbit

Isotype: IgG

Reserch Areas: Neuroscience, Isotype/Loading Controls, Signal Transduction, Cancer, Metabolism

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.

Concentration: 0.66 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein

Buffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3

Purification Method: Antigen Affinity Purification

Dilution: WB 1:3000-1:6000 IHC 1:300-1:500

Clone: N/A

Conjugation: Unconjugated

Molecular Weight(Calculated): 35 kDa

Molecular Weight(Observed): 35 kDa